- 01/01/2018 - 31/12/2018
- Centre aux Energies Atomiques (CEA), France
- Funded by
- EIT Health (Innovation by Ideas)
The increasing rates of infections caused by broad-spectrum β-lactamases (ESBLs and carbapenemases)-producing Enterobacteriaceae is a major public health concern.
To fight efficiently against the emergence and the spread of these multidrug (sometimes even pandrug) resistant bacteria, rapid detection and characterization is a prerequisite to implement proper hygiene and infection control measures, as well as a more adequate empirical treatment. Several methods already exist to detect antimicrobial resistance but these techniques require isolated bacteria and procedures with non-compatible delays (≥16 hours).
Thus, sensitive, rapid, low cost and user-friendly tests need to be implemented in clinical microbiology laboratories. Due to its characteristics the lateral flow immunoassay (LFIA) matches perfectly with these specifications.
The aim of this project is to validate a diagnostic tool using the LFIA technology for the detection of broad spectrum β-lactamases (CTXM) and carbapenemases (OXA; NDM; KPC, VIM, IMP) directy from biological samples.
We will develop a test consisting of a nitrocellulose strip, which allows the immunological detection, enclosed in a plastic device, which carries out sample treatment. The devices, produced by 3D printing, will be optimized and validated with real clinical samples.
The operational procedures will be improved to fit with clinical habits and analytical specifications (specificity, reproducibility and sensitivity) and a cost-effectiveness analysis will be carried out.
At the end of this project a business concept will be established and training tools for
end-users will be available to accelerate the industrial transfer of this technology and
its implementation in care facilities.
Other projectsSee Past Projects
Combating Bacterial Resistance In Europe
Application to medical deviceshttp://www.nomorfilm.eu
Combatting Bacterial Resistance in Europe - Carbapenem Resistancehttps://www.imi.europa.eu/content/combacte-care
Prioritising Antimicrobial Resistance: establishing an interdisciplinary International Research Partnership to tackle an evolving global health threat
An interdisciplinary International Research Partnership to tackle an evolving global health threat
Emergencia de variantes de citomegalovirus portadoras de mutaciones asociadas a resistencia en trasplantados de órgano sólido
Resistencia a los antivirales del citomegalovirus en el trasplante
Development of new metal-antibiotics against pathogens causing chronic infections in patients with cystic fibrosis.
New metal-antibiotics for patients with cystic fibrosis
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Surveillance of Zika virus infection in pregnant women travelling from affected areas
Non-invasive ultrasound-based screening for meningitis: Proof-of concept in infants
Search for new target proteins and use of antibacterial or inhibitory peptides against resistance mechanisms in 'Acinetobacter baumannii'
New approaches for designing antibacterial compounds against multiresistant gram-negative bacteria
Development of New Metalloantibiotics Against Pathogens Associated to Chronic Infections in Cystic Fibrosis Patients
Optimizing provider-initiated HIV testing, linkage, and retention in care in the district of Manhiça, Mozambique