Publicador de continguts



Portable Graphene Multiplex Electrochemical Sensor for Rapid Detection of Severe Malaria

Foto: Canva
01/07/2022 - 30/06/2025
Claudio Parolo
Ministerio de Ciencia e Innovación - Agencia Estatal De Investigación. Reference: CPP2021-008658 Call: Proyectos de colaboración público-privada

In 2020, malaria caused over 600,000 deaths and affected 241 million patients worldwide, representing a major healthcare and economic burden for many countries. In response to this the World Health Organization has established a malaria eradication program that has in the development of early-diagnostic tests one if its major pillars. In fact current gold standard tests for malaria diagnostics (microscopy and PCR) require fully-equipped laboratory, trained personnel and expensive equipment. In recent years, the use of lateral flow assays have revolutionized malaria diagnostics, allowing for a rapid response even at the point of care. However, they generally have lower sensitivity and specificity of the gold standard techniques, therefore requiring often a confirmatory analysis. Furthermore the vast majority of lateral flow assays detect the HRP2 protein, a malaria biomarker that is being deleted by the parasite in many endemic regions. This is generating a higher number of false negative results that represent a major threat for the patient’s wellbeing. 

In the G_SensMalaria project, we plan to develop a multiplexed, point-of-care, electrochemical biosensor for the quantitative detection of up to 5 diagnostic (HRP2 and LDH) and prognostic (ANG1, ANG2 and sTREM) malaria biomarkers. Specifically, our device relies on two main parts: an electrochemical aptamer-based (EAB) sensor using a graphene-based printed inks and a portable potentiostat reader. The use of EAB sensors allows us to quantify biomarkers in less than 5 minutes, directly in undiluted blood. This is a unique technology that combine the properties of point-of-care sensors (speed and ease of use) with those of laboratory-based ones (quantification). For the fabrication of the graphene-based electrodes we will use the patented technology of GraphenicaLab (called wax-printed membrane) that allows to quickly and cheaply print thousands of electrodes using pre-functionalized (in this case with aptamers) graphene and other nanomaterials. Finally, thanks to the experience of Eurecat in the development of electrical hardware, we will be able to create a portable potentiostat capable to quantify the signal from 5 different working electrode and send the results wirelessly to a smartphone. The final prototype will be able to quantify in a single drop of blood the five biomarkers allowing the medical personnel to both diagnose and prognose malaria within minutes

Indeed, this is an ambitious proposal that has the potential to really impact clinical practice in both developing and developed regions. We believe that our consortium is uniquely positioned to carry out such endevour, combining the clinical and biotechnological experience of the ISGlobal team, the chemistry and nanotechnological know how of Graphenicalab and the electrical and engineering capability of Eurecat, to deliver a disruptive fully working prototype ready to be validated and commercialized.

Total funding 

181,821 € 

Our Team


ISGlobal team

Altres projectes

Veure projectes passats


L'Aliança Científica per a l'Eradicació de la Malària (MESA) té com a objectiu avançar en la ciència de l'eradicació de la malària.


Noves Eines per al Diagnòstic i l'Avaluació del Pacient amb Malaltia de Chagas

RTS,S Vaccine Immunology Study

Study of immune correlates of protection against malaria after vaccination with RTS,S/AS01E: a comprehensive immunological arm of a Phase III double-blind, randomized, controlled multi-center trial

Control of Leishmaniasis. From bench to bedside and community


Grup de recerca en patología importada i malaties emergents i re-emergents


Nuevos regímenes de quimioterapia y biomarcadores para la enfermedad de Chagas


Broad One Health Endectocide-based Malaria Intervention in Africa


Unravelling Disease Tolerance and Host Resistance in Afebrile 'P. falciparum' Infections: a Prospective Study in Mozambican Adults


P. falciparum genomic intelligence in Mozambique


Administración masiva y focal de fármacos antimaláricos para avanzar hacia la eliminación de la malaria en Mozambique: acelerando la implementación de programas y políticas


MULTIple doses of IPTi Proposal: a Lifesaving high Yield intervention


Citizens engagement digital platform for collective intelligence in pandemics


Novel organ-on-a-chip technology to study extracellular vesicles-mediated cryptic infections in Plasmodium vivax malaria

MENA Migrant Health

Transforming data collection and surveillance to drive migrant health research, care and policy


Social affairs and sex in P. falciparum: implications for malaria elimination


Building Scalable Pathogen Genomic Epidemiology in Ethiopia


Identifying Severe Malaria with a new Aptamer-based Rapid diagnostic Test