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Evaluating a new stool based qPCR for diagnosis of tuberculosis in children and people living with HIV

Logo del proyecto Stool4TB
Foto: Cristina Castellana
01/10/2020 - 01/10/2024
Alberto García-Basteiro

STool4TB is a project that aims to validate an innovative stool homogenization and DNA isolation method that yields a highly sensitive and specific Mtb qPCR-based diagnostic. STool4TB will evaluate this platform in the high TB and HIV burden settings of Mozambique, Eswatini and Uganda, under the hypothesis that it will narrow the large TB case detection gap by improving TB confirmation rates in children and PLHIV, while proving feasible and acceptable.

Preliminary data suggest that this platform adds value to existing sputum based diagnostics and increases rates of bacteriological confirmation. Given the lack of available tools to effectively monitor TB treatment, STool4TB will also evaluate the usefulness of this quantitative stool-based qPCR as a TB treatment monitoring tool for children and PLHIV.

This platform has the potential to be adapted to a POC diagnostic and thus easily implemented in resource-constrained basic health care centres. This proposal represents an opportunity to assess the feasibility, usability and acceptability of stool collection from both the patient and the health system perspective. STool4TB will create a biobank of comparative specimens provided by pediatric and HIV-positive TB patients (as well as TB-free individuals). The biobank will facilitate future evaluation of novel assays for pediatric TB diagnosis or drug resistance detection.

Finally, we aim to create a TB diagnostic network among African high TB burden countries with the capacity to conduct clinical studies of novel diagnostics and new drugs with a focus on children and PLHIV. This will be achieved by strengthening collaboration and sustaining capacity building activities among participating African institutions.

Total Funding

€ 2,999,989.25

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ISGlobal Team

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